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Anti-Synaptophysin/SYP Antibody (1U942)
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Catalog No. TMAC-03936
Anti-Synaptophysin/SYP Antibody (1U942) is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Antibody (1U942) can be used in WB,ICC/IF,IHC.
Anti-Synaptophysin/SYP Antibody (1U942)
Anti-Synaptophysin/SYP Antibody (1U942)
😃Good
Catalog No. TMAC-03936
Anti-Synaptophysin/SYP Antibody (1U942) is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Antibody (1U942) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-Synaptophysin/SYP Antibody (1U942) is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Antibody (1U942) can be used in WB,ICC/IF,IHC. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution. Positive control: Lane 1: Human brain, Lane 2: PC-12. 2. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin. 5. ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis. In addition, the tail domain of brain Myosin V also forms a stable complex with synaptobrevin II and SYP, and this complex is disassembled upon the depolarization-induced entry of Ca2+ into intact nerve endings. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 38 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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