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Anti-STAT3 Antibody (7C950)
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Catalog No. TMAC-03875
Alias signal transducer and activator of transcription 3 (acute-phase response factor), HIES, APRF, ADMIO
Anti-STAT3 Antibody (7C950) is a Rabbit antibody targeting STAT3. Anti-STAT3 Antibody (7C950) can be used in WB,ICC/IF,IHC.
Anti-STAT3 Antibody (7C950)
Anti-STAT3 Antibody (7C950)
😃Good
Catalog No. TMAC-03875Alias signal transducer and activator of transcription 3 (acute-phase response factor), HIES, APRF, ADMIO
Anti-STAT3 Antibody (7C950) is a Rabbit antibody targeting STAT3. Anti-STAT3 Antibody (7C950) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-STAT3 Antibody (7C950) is a Rabbit antibody targeting STAT3. Anti-STAT3 Antibody (7C950) can be used in WB,ICC/IF,IHC. |
| Synonyms | signal transducer and activator of transcription 3 (acute-phase response factor), HIES, APRF, ADMIO |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of STAT3 on MCF-7 cells lysates using anti-STAT3 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-STAT3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-STAT3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-STAT3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-STAT3 antibody. Counter stained with hematoxylin. 6. ICC staining STAT3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining STAT3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining STAT3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 88 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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