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Anti-SPI1 Antibody (2J454)
😃Good
Catalog No. TMAC-03470
Alias Transcription factor PU.1, SPI1, 31 kDa-transforming protein
Anti-SPI1 Antibody (2J454) is a Rabbit antibody targeting SPI1. Anti-SPI1 Antibody (2J454) can be used in FCM,IHC,WB.
Anti-SPI1 Antibody (2J454)
Anti-SPI1 Antibody (2J454)
😃Good
Catalog No. TMAC-03470Alias Transcription factor PU.1, SPI1, 31 kDa-transforming protein
Anti-SPI1 Antibody (2J454) is a Rabbit antibody targeting SPI1. Anti-SPI1 Antibody (2J454) can be used in FCM,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SPI1 Antibody (2J454) is a Rabbit antibody targeting SPI1. Anti-SPI1 Antibody (2J454) can be used in FCM,IHC,WB. |
| Synonyms | Transcription factor PU.1, SPI1, 31 kDa-transforming protein |
| Ig Type | IgG |
| Clone | 2J454 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of PU.1/Spi1 on THP-1 cell using anti-PU.1/Spi1 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin. 4. Flow cytometric analysis of K562 cells with PU.1/Spi1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.     |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The Ets transcription factor family (Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T-cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T-lineage commitment and then is dramatically absent following the beta-selection checkpoint. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 31 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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