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Anti-SPARCL1 Polyclonal Antibody
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Catalog No. TMAB-01765
Alias SPARC like 1, Sc1, mast9, hevin, Ecm2
Anti-SPARCL1 Polyclonal Antibody is a Rabbit antibody targeting SPARCL1. Anti-SPARCL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-SPARCL1 Polyclonal Antibody
Anti-SPARCL1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01765Alias SPARC like 1, Sc1, mast9, hevin, Ecm2
Anti-SPARCL1 Polyclonal Antibody is a Rabbit antibody targeting SPARCL1. Anti-SPARCL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-SPARCL1 Polyclonal Antibody is a Rabbit antibody targeting SPARCL1. Anti-SPARCL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | SPARC like 1, Sc1, mast9, hevin, Ecm2 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Pig,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (SPARCL1) Polyclonal Antibody, Unconjugated (TMAB-01765) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-SPARCL1 (TMAB-01765) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 73 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Tissue Specificity | Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | SPARC (secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC-like protein 1 (SPARCL1), also known as high endothelial venule protein (Hevin) or MAST9, is a 664 amino acid member of the SPARC family of proteins. Highly expressed in lymph node, heart, lung, brain, skeletal muscle, ovary, colon and small intestine, SPARCL1 is a secreted protein that contains one EF-hand domain, one follistatin-like domain and one Kazal-like domain. SPARCL1 is implicated to play a role in neuronal remodeling and tumor suppression. The gene encoding SPARCL1 maps to chromosome 4q22.1. |
| Immunogen | KLH conjugated synthetic peptide: human SPARCL1/Ecm2 |
| Antigen Species | Human |
| Gene Name | SPARCL1 |
| Gene ID | |
| Protein Name | SPARC-like protein 1 |
| Uniprot ID | |
| Biology Area | Tumor suppressors,Tumor biomarkers |
| Molecular Weight | Theoretical: 74 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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