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Anti-SPARCL1 Polyclonal Antibody
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Catalog No. TMAB-01765
Alias SPARC like 1, Sc1, mast9, hevin, Ecm2
Anti-SPARCL1 Polyclonal Antibody is a Rabbit antibody targeting SPARCL1. Anti-SPARCL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-SPARCL1 Polyclonal Antibody
Anti-SPARCL1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01765Alias SPARC like 1, Sc1, mast9, hevin, Ecm2
Anti-SPARCL1 Polyclonal Antibody is a Rabbit antibody targeting SPARCL1. Anti-SPARCL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-SPARCL1 Polyclonal Antibody is a Rabbit antibody targeting SPARCL1. Anti-SPARCL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | SPARC like 1, Sc1, mast9, hevin, Ecm2 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Pig,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (SPARCL1) Polyclonal Antibody, Unconjugated (TMAB-01765) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-SPARCL1 (TMAB-01765) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 73 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | SPARC (secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC-like protein 1 (SPARCL1), also known as high endothelial venule protein (Hevin) or MAST9, is a 664 amino acid member of the SPARC family of proteins. Highly expressed in lymph node, heart, lung, brain, skeletal muscle, ovary, colon and small intestine, SPARCL1 is a secreted protein that contains one EF-hand domain, one follistatin-like domain and one Kazal-like domain. SPARCL1 is implicated to play a role in neuronal remodeling and tumor suppression. The gene encoding SPARCL1 maps to chromosome 4q22.1. |
| Immunogen | KLH conjugated synthetic peptide: human SPARCL1/Ecm2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Tumor suppressors,Tumor biomarkers |
| Molecular Weight | Theoretical: 74 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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