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Anti-SPARCL1 Antibody-PE (5B937)
🥰Excellent
Catalog No. TMAY-00164P
Anti-SPARCL1 Antibody-PE (5B937) is a PE-conjugated Mouse antibody targeting SPARCL1. Anti-SPARCL1 Antibody-PE (5B937) can be used in FCM.
Anti-SPARCL1 Antibody-PE (5B937)
Anti-SPARCL1 Antibody-PE (5B937)
🥰Excellent
Catalog No. TMAY-00164P
Anti-SPARCL1 Antibody-PE (5B937) is a PE-conjugated Mouse antibody targeting SPARCL1. Anti-SPARCL1 Antibody-PE (5B937) can be used in FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SPARCL1 Antibody-PE (5B937) is a PE-conjugated Mouse antibody targeting SPARCL1. Anti-SPARCL1 Antibody-PE (5B937) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 5B937 |
| Reactivity | Human |
| Verified Activity | Profile of anti-SPARCL1 reactivity on HL60 cells analyzed by flow cytometry. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with PE conjugated Mouse anti-SPARCL1, The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM |
| Recommended Dose | 5 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SPARCL1 (rh SPARCL1; NP_004675.3Met 1-Phe 664) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Research Background | SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. Besides, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS). |
| Conjucates | PE |
| Others Formats | Unconjugated/FITC |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human SPARCL1 protein |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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