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Anti-SMAD2 Polyclonal Antibody is a Rabbit antibody targeting SMAD2. Anti-SMAD2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-SMAD2 Polyclonal Antibody is a Rabbit antibody targeting SMAD2. Anti-SMAD2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Rabbit) |
Verified Activity | 1. Tissue/cell: rat skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Smad2 Polyclonal Antibody, Unconjugated (TMAB-01736) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat choroid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Smad2 Polyclonal Antibody, Unconjugated (TMAB-01736) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Heart (Mouse) Lysate at 40 μg Lane 3: Testis (Mouse) Lysate at 40 μg Lane 4: Kidney (Mouse) Lysate at 40 μg Lane 5: Placenta (Mouse) Lysate at 40 μg Lane 6: Cerebrum (Rat) Lysate at 40 μg Lane 7: Heart (Rat) Lysate at 40 μg Lane 8: Testis (Rat) Lysate at 40 μg Lane 9: Kidney (Rat) Lysate at 40 μg Lane 10: Hela (Human) Cell Lysate at 30 μg Lane 11: HT1080 (Human) Cell Lysate at 30 μg Lane 12: Jurkat (Human) Cell Lysate at 30 μg Lane 13: Raw264.7 (Mouse) Cell Lysate at 30 μg Lane 14: HL60 (Human) Cell Lysate at 30 μg Primary: Anti-Smad2 (TMAB-01736) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa 4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Smad2) Polyclonal Antibody, Unconjugated (TMAB-01736) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Blank control (Black line): Mouse spleen (Black). Primary Antibody (green line): Rabbit Anti-Smad2 antibody (TMAB-01736) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012] |
Immunogen | KLH conjugated synthetic peptide: human Smad2 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | TGF,SMADs,SMADs,Cytoplasmic |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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