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Anti-SIN3A Antibody (9Z567)
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Catalog No. TMAC-02653
Alias Transcriptional corepressor Sin3a, Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a
Anti-SIN3A Antibody (9Z567) is a Rabbit antibody targeting SIN3A. Anti-SIN3A Antibody (9Z567) can be used in WB,ICC/IF,IHC,FCM.
Anti-SIN3A Antibody (9Z567)
Anti-SIN3A Antibody (9Z567)
😃Good
Catalog No. TMAC-02653Alias Transcriptional corepressor Sin3a, Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a
Anti-SIN3A Antibody (9Z567) is a Rabbit antibody targeting SIN3A. Anti-SIN3A Antibody (9Z567) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-SIN3A Antibody (9Z567) is a Rabbit antibody targeting SIN3A. Anti-SIN3A Antibody (9Z567) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | Transcriptional corepressor Sin3a, Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of mSin3A on 293T cell using anti-mSin3A antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-mSin3A antibody. Counter stained with hematoxylin. 3. ICC staining mSin3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining mSin3A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining mSin3A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of MCF-7 cells with mSin3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (alternatively designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad-binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 145 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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