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Anti-S100A9 Antibody-PE (7L821)

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Catalog No. TMAY-01695P

Anti-S100A9 Antibody-PE (7L821) is a PE-conjugated Mouse antibody targeting S100A9. Anti-S100A9 Antibody-PE (7L821) can be used in FCM.

Anti-S100A9 Antibody-PE (7L821)

Anti-S100A9 Antibody-PE (7L821)

🥰Excellent
Catalog No. TMAY-01695P
Anti-S100A9 Antibody-PE (7L821) is a PE-conjugated Mouse antibody targeting S100A9. Anti-S100A9 Antibody-PE (7L821) can be used in FCM.
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Product Introduction

Bioactivity
Description
Anti-S100A9 Antibody-PE (7L821) is a PE-conjugated Mouse antibody targeting S100A9. Anti-S100A9 Antibody-PE (7L821) can be used in FCM.
Ig Type
Monoclonal Mouse IgG2b
Clone
7L821
Reactivity
Human
Verified Activity
Profile of anti-S100A9 reactivity on MCF-7 cells analyzed by flow cytometry. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with PE conjugated Mouse anti-Human S100A9, The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
FCM
Recommended Dose
1 μl/Test, 0.1 mg/ml
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A9 (rh S100A9; TMPY-01357; NP_002956.1; Met1-Pro114) and conjugated with PE under optimum conditions, the unreacted PE was removed.
PurificationProtein A
AppearanceLiquid
FormulationPBS solution containing 0.5% BSA and 0.03%ProClin300
Research BackgroundS100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A9, also known as S100 calciumbinding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a proinflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.
Related Conjugates and Formulations
Conjucates
PE
Others Formats
Unconjugated/FITC
Antibody Types Available
3
Antigen Details
Immunogen
Recombinant Human S100A9 protein (TMPY-01357)
Antigen Species
Human
Biology Area
Calcium-binding Proteins and Related Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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