Anti-RPA70 Polyclonal Antibody is a Rabbit antibody targeting RPA70. Anti-RPA70 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Rabbit)

Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-RPA70 antibody (TMAB-12349)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human RPA70

Human

RPA1

Replication protein A 70 kDa DNA-binding subunit

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.