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Anti-ROR alpha Polyclonal Antibody

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Catalog No. TMAB-01629

Anti-ROR alpha Polyclonal Antibody is a Rabbit antibody targeting ROR alpha. Anti-ROR alpha Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ROR alpha Polyclonal Antibody

Anti-ROR alpha Polyclonal Antibody

😃Good
Catalog No. TMAB-01629
Anti-ROR alpha Polyclonal Antibody is a Rabbit antibody targeting ROR alpha. Anti-ROR alpha Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
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Product Introduction

Bioactivity
Description
Anti-ROR alpha Polyclonal Antibody is a Rabbit antibody targeting ROR alpha. Anti-ROR alpha Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Human HeLa cell lysates
Primary: Anti-ROR alpha (TMAB-01629) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ROR alpha) Polyclonal Antibody, Unconjugated (TMAB-01629) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
FormulationPreservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
Research BackgroundThe protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse ROR alpha
Antigen Species
Mouse
Biology Area
Retanoic and retanoid,Cell differentiation,Cell Differentiation,Retinoic & Retinoid,Neural Signal Transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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