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Anti-ROR alpha Polyclonal Antibody
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Catalog No. TMAB-01629
Alias RZR-ALPHA, RZRA, RORα, RORA, Rora, ROR α, Retinoid-related orphan receptor-alpha, RAR-related orphan receptor A, Nuclear receptor subfamily 1 group F member 1, Nuclear receptor RZR-alpha, Nuclear receptor ROR-alpha, NR1F1
Anti-ROR alpha Polyclonal Antibody is a Rabbit antibody targeting ROR alpha. Anti-ROR alpha Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ROR alpha Polyclonal Antibody
Anti-ROR alpha Polyclonal Antibody
😃Good
Catalog No. TMAB-01629Alias RZR-ALPHA, RZRA, RORα, RORA, Rora, ROR α, Retinoid-related orphan receptor-alpha, RAR-related orphan receptor A, Nuclear receptor subfamily 1 group F member 1, Nuclear receptor RZR-alpha, Nuclear receptor ROR-alpha, NR1F1
Anti-ROR alpha Polyclonal Antibody is a Rabbit antibody targeting ROR alpha. Anti-ROR alpha Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ROR alpha Polyclonal Antibody is a Rabbit antibody targeting ROR alpha. Anti-ROR alpha Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | RZR-ALPHA, RZRA, RORα, RORA, Rora, ROR α, Retinoid-related orphan receptor-alpha, RAR-related orphan receptor A, Nuclear receptor subfamily 1 group F member 1, Nuclear receptor RZR-alpha, Nuclear receptor ROR-alpha, NR1F1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Rat Heart tissue lysates Lane 3: Human HeLa cell lysates Primary: Anti-ROR alpha (TMAB-01629) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ROR alpha) Polyclonal Antibody, Unconjugated (TMAB-01629) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | Widely expressed in a number of tissues. Expressed in both regulatory T-cells (Treg) and effector T-cells (Teff). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Concentration | 1 mg/mL |
| Research Background | The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014] |
| Immunogen | KLH conjugated synthetic peptide: mouse ROR alpha |
| Antigen Species | Mouse |
| Gene Name | RORA |
| Protein Name | Nuclear receptor ROR-alpha |
| Biology Area | Retanoic and retanoid,Cell differentiation,Cell Differentiation,Retinoic & Retinoid,Neural Signal Transduction |
| Function | Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. |
| Molecular Weight | Theoretical: 59 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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