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Anti-RIPK2 Polyclonal Antibody
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Catalog No. TMAB-01621
Alias Tyrosine-protein kinase RIPK2, RIP-like-interacting CLARP kinase, RIPK2, RIP2, RICK, Receptor-interacting serine/threonine-protein kinase 2, Receptor-interacting protein 2 (RIP-2), CARDIAK, CARD-containing interleukin-1 beta-converting enzyme-associated kinase (CARD-containing IL-1 beta ICE-kinase)
Anti-RIPK2 Polyclonal Antibody is a Rabbit antibody targeting RIPK2. Anti-RIPK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-RIPK2 Polyclonal Antibody
Anti-RIPK2 Polyclonal Antibody
😃Good
Catalog No. TMAB-01621Alias Tyrosine-protein kinase RIPK2, RIP-like-interacting CLARP kinase, RIPK2, RIP2, RICK, Receptor-interacting serine/threonine-protein kinase 2, Receptor-interacting protein 2 (RIP-2), CARDIAK, CARD-containing interleukin-1 beta-converting enzyme-associated kinase (CARD-containing IL-1 beta ICE-kinase)
Anti-RIPK2 Polyclonal Antibody is a Rabbit antibody targeting RIPK2. Anti-RIPK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-RIPK2 Polyclonal Antibody is a Rabbit antibody targeting RIPK2. Anti-RIPK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | Tyrosine-protein kinase RIPK2, RIP-like-interacting CLARP kinase, RIPK2, RIP2, RICK, Receptor-interacting serine/threonine-protein kinase 2, Receptor-interacting protein 2 (RIP-2), CARDIAK, CARD-containing interleukin-1 beta-converting enzyme-associated kinase (CARD-containing IL-1 beta ICE-kinase) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,GuineaPig) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RIP2) Polyclonal Antibody, Unconjugated (TMAB-01621) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Hela (Human) Cell Lysate at 30 μg Primary: Anti-RIP2 (TMAB-01621) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa 3. Blank control: A431. Primary Antibody (green line): Rabbit Anti-RIP2 antibody (TMAB-01621) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human RIP2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Keywords
RIPK 2RIP-2RIP 2CARD-containing interleukin-1 β-converting enzyme-associated kinase (CARD-containing IL-1 β ICE-kinase)CARD-containing interleukin-1 b-converting enzyme-associated kinase (CARD-containing IL-1 b ICE-kinase)CARD-containing IL-1 β ICE-kinaseCARD-containing IL-1 beta ICE-kinaseCARD-containing IL-1 b ICE-kinase
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