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Anti-RhoA Antibody-PE (1W191)
🥰Excellent
Catalog No. TMAY-02348P
Anti-RhoA Antibody-PE (1W191) is a PE-conjugated Mouse antibody targeting RhoA. Anti-RhoA Antibody-PE (1W191) can be used in FCM.
Anti-RhoA Antibody-PE (1W191)
Anti-RhoA Antibody-PE (1W191)
🥰Excellent
Catalog No. TMAY-02348P
Anti-RhoA Antibody-PE (1W191) is a PE-conjugated Mouse antibody targeting RhoA. Anti-RhoA Antibody-PE (1W191) can be used in FCM.
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Product Introduction
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Related Conjugates and Formulations
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Chemical Properties
| Description | Anti-RhoA Antibody-PE (1W191) is a PE-conjugated Mouse antibody targeting RhoA. Anti-RhoA Antibody-PE (1W191) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG2a |
| Clone | 1W191 |
| Reactivity | Human |
| Verified Activity | Profile of anti-RHOA reactivity on HeLa cells analyzed by flow cytometry. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with PE conjugated Mouse anti-RHOA, The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM |
| Recommended Dose | 10 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RhoA (rh RhoA; TMPY-01958; P61586-1; Met 1-Leu 193) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Research Background | Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein that belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. |
| Conjucates | PE |
| Others Formats | Unconjugated |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Human RhoA protein (TMPY-01958) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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