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Anti-RhoA Antibody-FITC (4W246)
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Catalog No. TMAY-02350F
Alias RHOH12, RHO12, ras homolog family member A, ARHA, ARH12
Anti-RhoA Antibody-FITC (4W246) is a FITC-conjugated Rabbit antibody targeting RhoA. Anti-RhoA Antibody-FITC (4W246) can be used in FCM.
Anti-RhoA Antibody-FITC (4W246)
Anti-RhoA Antibody-FITC (4W246)
😃Good
Catalog No. TMAY-02350FAlias RHOH12, RHO12, ras homolog family member A, ARHA, ARH12
Anti-RhoA Antibody-FITC (4W246) is a FITC-conjugated Rabbit antibody targeting RhoA. Anti-RhoA Antibody-FITC (4W246) can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 T | $104 | 7-10 days | |
| 100 T | $211 | 7-10 days |
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Chemical Properties
| Description | Anti-RhoA Antibody-FITC (4W246) is a FITC-conjugated Rabbit antibody targeting RhoA. Anti-RhoA Antibody-FITC (4W246) can be used in FCM. |
| Alias | RHOH12, RHO12, ras homolog family member A, ARHA, ARH12 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 4W246 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of Human RHOA expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Rabbit anti-Human RHOA. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM |
| Recommended Dose | 10 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human RhoA (rh RhoA; TMPY-01958; P61586-1; Met1-Leu193) and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Research Background | Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein that belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. |
| Conjucates | FITC |
| Others Formats | Unconjugated |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Human RhoA protein (TMPY-01958) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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