Anti-PIGR Polyclonal Antibody is a Rabbit antibody targeting PIGR. Anti-PIGR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PIGR) Polyclonal Antibody, Unconjugated (TMAB-01537) at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 minu
2. Sample:
293t (Human) Cell Lysate at 30 μg
Primary: Anti-PIGR (TMAB-01537) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 131 kDa
3. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-PIGR (TMAB-01537) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 131 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PIGR

Human

PIGR

Polymeric immunoglobulin receptor

Receptors

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.