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Anti-Phospho-Tyk2 (Tyr1054, 1055) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Tyk2 (Tyr1054, 1055). Anti-Phospho-Tyk2 (Tyr1054, 1055) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Phospho-Tyk2 (Tyr1054, 1055) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Tyk2 (Tyr1054, 1055). Anti-Phospho-Tyk2 (Tyr1054, 1055) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | Tyk2 (p-Y1054, 1055), Tyk2 (p-Tyr1054, 1055), TYK2, p-Tyk2 (Y1054, 1055), p-Tyk2 (Tyr1054, 1055), Non-receptor tyrosine-protein kinase TYK2 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog) |
Verified Activity | 1. Sample: A549 (Human) Cell Lysate at 30 μg Primary: Anti-Phospho-Tyk2 (Tyr1054 + Tyr1055) (TMAB-01526) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 134 kDa Observed band size: 134 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Phospho-Tyk2 (Tyr1054 + Tyr1055)) Polyclonal Antibody, Unconjugated (TMAB-01526) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Phospho-Tyk2 (Tyr1054 + Tyr1055)) Polyclonal Antibody, Unconjugated (TMAB-01526) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Tyk2 (Tyr1054 + Tyr1055)) Polyclonal Antibody, Unconjugated (TMAB-01526) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with hyperimmunoglobulin E syndrome (HIES) - a primary immunodeficiency characterized by elevated serum immunoglobulin E. [provided by RefSeq]. |
Immunogen | KLH conjugated Synthesised phosphopeptide: human Tyk2 around the phosphorylation site of Tyr1054/1055 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | SARS Coronavirus,Tyrosine Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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