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Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody

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Catalog No. TMAB-01433
Alias p-IRE1 (Ser726), p-IRE1 (S726), IRE1P, IRE1a, IRE1 (p-Ser726), IRE1 (p-S726), IRE1, hIRE1p, endoplasmic reticulum to nucleus signaling 1

Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRE1 (Ser726). Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody

Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody

😃Good
Catalog No. TMAB-01433Alias p-IRE1 (Ser726), p-IRE1 (S726), IRE1P, IRE1a, IRE1 (p-Ser726), IRE1 (p-S726), IRE1, hIRE1p, endoplasmic reticulum to nucleus signaling 1
Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRE1 (Ser726). Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRE1 (Ser726). Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Aliasp-IRE1 (Ser726), p-IRE1 (S726), IRE1P, IRE1a, IRE1 (p-Ser726), IRE1 (p-S726), IRE1, hIRE1p, endoplasmic reticulum to nucleus signaling 1
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRE1a (Ser 726)) Polyclonal Antibody, Unconjugated (TMAB-01433) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-phospho-IRE1a (Ser 726) antibody (TMAB-01433)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundERN2, endoplasmic reticulum to nucleus signaling 2, induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. It is pro-apoptotic and appears to play no role in the unfolded-protein response, unlike closely related proteins.
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human IRE1a around the phosphorylation site of Ser726
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Other Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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