Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody
😃Good
Catalog No. TMAB-01400
Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Cofilin (Ser3). Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody
Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody
😃Good
Catalog No. TMAB-01400
Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Cofilin (Ser3). Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Cofilin (Ser3). Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (TMAB-01400) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (TMAB-01400) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-Phospho-Cofilin (Ser3) (TMAB-01400) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human Cofilin around the phosphorylation site of Ser3 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cell adhesion,Actins,Cytoskeleton,Axon marker,Axonal Guidance Proteins,Actin Binding Proteins |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Sci Citations
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody | purchase Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody | Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody cost | order Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.