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Anti-PDK4 Polyclonal Antibody
😃Good
Catalog No. TMAB-01358
Alias pyruvate dehydrogenase kinase 4, AV005916
Anti-PDK4 Polyclonal Antibody is a Rabbit antibody targeting PDK4. Anti-PDK4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PDK4 Polyclonal Antibody
Anti-PDK4 Polyclonal Antibody
😃Good
Catalog No. TMAB-01358Alias pyruvate dehydrogenase kinase 4, AV005916
Anti-PDK4 Polyclonal Antibody is a Rabbit antibody targeting PDK4. Anti-PDK4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PDK4 Polyclonal Antibody is a Rabbit antibody targeting PDK4. Anti-PDK4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | pyruvate dehydrogenase kinase 4, AV005916 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PDK4) Polyclonal Antibody, Unconjugated (TMAB-01358) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PDK4) Polyclonal Antibody, Unconjugated (TMAB-01358) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-PDK4 (TMAB-01358) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 45 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion matrix. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Pdk4 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Energy Metabolism,Mitochondrial markers,Cancer,Energy Metabolism,Mitochondrial |
| Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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