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Anti-PDK1 Antibody (2G768) (Synonyms: pyruvate dehydrogenase kinase, isozyme 1, PDHK1)

Catalog No. TMAC-03138 Copy Product Info
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Anti-PDK1 Antibody (2G768) is a Rabbit antibody targeting PDK1. Anti-PDK1 Antibody (2G768) can be used in FCM,IHC,IP,WB.

Anti-PDK1 Antibody (2G768)

Copy Product Info
🥰Excellent
Catalog No. TMAC-03138
Synonyms pyruvate dehydrogenase kinase, isozyme 1, PDHK1

Anti-PDK1 Antibody (2G768) is a Rabbit antibody targeting PDK1. Anti-PDK1 Antibody (2G768) can be used in FCM,IHC,IP,WB.

Anti-PDK1 Antibody
(2G768)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-PDK1 Antibody (2G768) is a Rabbit antibody targeting PDK1. Anti-PDK1 Antibody (2G768) can be used in FCM,IHC,IP,WB.
Synonymspyruvate dehydrogenase kinase, isozyme 1, PDHK1
Ig Type
IgG
Clone
2G768
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of PDK1 on rat heart tissue lysate using anti-PDK1 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDK1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PDK1 antibody. Counter stained with hematoxylin.
4. ICC staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining PDK1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of NIH-3T3 cells with PDK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; ICC:1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundMitochondrial pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate and plays a central role in the regulation of homeostasis of carbohydrate fuels in mammals. PDH activity is controlled by a phosphorylation/dephosphorylation cycle, phosphorylation leading to inactivation and dephosphorylation leading to reactivation of PDH. The phosphorylation of PDH is catalyzed by pyruvate dehydrogenase kinase (PDK), the activity of which is stimulated by the products of PDH catalysis. PDK1 consists of alpha and beta subunits; the kinase activity resides in the alpha subunit. Three PDK isoenzymes have been identified in humans (PDK1, 2 and 3) and two have been identified in rodent (PDK1 and 2).
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 49 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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