Anti-PDCD6IP Polyclonal Antibody is a Rabbit antibody targeting PDCD6IP. Anti-PDCD6IP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Testis (Rat) Lysate at 40 μg
Lane 4: Cerebrum (Rat) Lysate at 40 μg
Lane 5: Jurkat (Human) Cell Lysate at 30 μg
Primary: Anti-ALIX (TMAB-01348) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 100 kDa
3. 25 μg total protein per Lane of various lysates probed with PDCD6IP polyclonal antibody, unconjugated (TMAB-01348) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PDCD6IP

Human

Death Ligands,Host Virus Interaction,Regulation