Anti-PD-L1 Polyclonal Antibody 4 is a Rabbit antibody targeting PD-L1. Anti-PD-L1 Polyclonal Antibody 4 can be used in FCM,WB.

IgG

Human,Mouse (predicted:Rat)

1. Sample:
Eye (Mouse) Lysate at 40 μg
LympHnode (Mouse) Lysate at 40 μg
Primary: Anti-PD-L1 (TMAB-01362) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa
2. Blank control (black line): RAW264.7. Primary Antibody (green line): Rabbit Anti-PD-L1 antibody (TMAB-01362)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: mouse PD-L1

Mouse

CD274

Programmed cell death 1 ligand 1

Tumor antigens,Tumor-associated antigens,Non-lineage,Autoimmune,pd-l1

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.