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Anti-PCNA Antibody (8X645)
😃Good
Catalog No. TMAC-03098
Alias proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (8X645) is a Mouse antibody targeting PCNA. Anti-PCNA Antibody (8X645) can be used in WB,IHC,FCM.
Anti-PCNA Antibody (8X645)
Anti-PCNA Antibody (8X645)
😃Good
Catalog No. TMAC-03098Alias proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (8X645) is a Mouse antibody targeting PCNA. Anti-PCNA Antibody (8X645) can be used in WB,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $426 | 7-10 days |
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Chemical Properties
| Description | Anti-PCNA Antibody (8X645) is a Mouse antibody targeting PCNA. Anti-PCNA Antibody (8X645) can be used in WB,IHC,FCM. |
| Synonyms | proliferating cell nuclear antigen, ATLD2 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PCNA on different cell lysates using anti-PCNA antibody at 1/1000 dilution. Positive control: Line 1: L929, Line 2: MCF-7, Line 3: PC12, Line 4: Raji, Line 5: F9, Line 6: A549. 2. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PCNA antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-PCNA antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of Hela cells with PCNA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:200; FCM: 1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | PCNA (Proliferating cell nuclear antigen) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. It induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 29 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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