Anti-ODC1 Polyclonal Antibody is a Rabbit antibody targeting ODC1. Anti-ODC1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse)

1. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (TMAB-01282) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample: Kidney (Mouse) lysate at 30 μg;
Primary: Anti-ODc (TMAB-01282) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size:51 kDa
Observed band size:51 kDa
3. Sample: Liver (Mouse) lysate at 30 μg;
Primary: Anti-ODc (TMAB-01282) at 1:300 dilution;
Secondary: AP conjugated Goat Anti-Rabbit Igg at 1: 5000 dilution;
Predicted band size: 51 kDa
Observed band size: 51 kDa
4. Sample: Kidney (Mouse) lysate at 30 μg;
Primary: Anti-ODc (TMAB-01282) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size:51 kDa
Observed band size:51 kDa
5. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (TMAB-01282) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ODC1

Human

ODC1

Ornithine decarboxylase

Amino acid metabolism,Amino Acids