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Anti-Nucleostemin Antibody (4J11)

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Catalog No. TMAC-02898
Alias Putative nucleotide binding protein estradiol induced, Nucleostemin, Nucleolar GTP-binding protein 3, Nucleolar GTP binding protein 3, NS, Novel nucleolar protein 47, NNP47, MGC800, Guanine nucleotide-binding protein-like 3, Guanine nucleotide binding protein like 3 (nucleolar), Guanine nucleotide binding protein like 3, GNL3_HUMAN, GNL3, Estradiol induced nucleotide binding protein, E2-induced gene 3 protein, E2IG3, E2 induced gene 3 protein, C77032

Anti-Nucleostemin Antibody (4J11) is a Rabbit antibody targeting Nucleostemin. Anti-Nucleostemin Antibody (4J11) can be used in WB,ICC,IHC,FCM.

Anti-Nucleostemin Antibody (4J11)

Anti-Nucleostemin Antibody (4J11)

😃Good
Catalog No. TMAC-02898Alias Putative nucleotide binding protein estradiol induced, Nucleostemin, Nucleolar GTP-binding protein 3, Nucleolar GTP binding protein 3, NS, Novel nucleolar protein 47, NNP47, MGC800, Guanine nucleotide-binding protein-like 3, Guanine nucleotide binding protein like 3 (nucleolar), Guanine nucleotide binding protein like 3, GNL3_HUMAN, GNL3, Estradiol induced nucleotide binding protein, E2-induced gene 3 protein, E2IG3, E2 induced gene 3 protein, C77032
Anti-Nucleostemin Antibody (4J11) is a Rabbit antibody targeting Nucleostemin. Anti-Nucleostemin Antibody (4J11) can be used in WB,ICC,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-Nucleostemin Antibody (4J11) is a Rabbit antibody targeting Nucleostemin. Anti-Nucleostemin Antibody (4J11) can be used in WB,ICC,IHC,FCM.
SynonymsPutative nucleotide binding protein estradiol induced, Nucleostemin, Nucleolar GTP-binding protein 3, Nucleolar GTP binding protein 3, NS, Novel nucleolar protein 47, NNP47, MGC800, Guanine nucleotide-binding protein-like 3, Guanine nucleotide binding protein like 3 (nucleolar), Guanine nucleotide binding protein like 3, GNL3_HUMAN, GNL3, Estradiol induced nucleotide binding protein, E2-induced gene 3 protein, E2IG3, E2 induced gene 3 protein, C77032
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Nucleostemin antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Nucleostemin antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Nucleostemin antibody. Counter stained with hematoxylin.
4. ICC staining Nucleostemin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Nucleostemin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of A549 cells with Nucleostemin antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: the C-terminus of human Nucleostemin
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 62 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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