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Anti-NR1I2 Polyclonal Antibody

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Catalog No. TMAB-01265
Alias Steroid and xenobiotic receptor (SXR), PXR, Pregnane X receptor, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, Nuclear receptor subfamily 1 group I member 2, NR1I2

Anti-NR1I2 Polyclonal Antibody is a Rabbit antibody targeting NR1I2. Anti-NR1I2 Polyclonal Antibody can be used in WB,FCM.

Anti-NR1I2 Polyclonal Antibody

Anti-NR1I2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01265Alias Steroid and xenobiotic receptor (SXR), PXR, Pregnane X receptor, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, Nuclear receptor subfamily 1 group I member 2, NR1I2
Anti-NR1I2 Polyclonal Antibody is a Rabbit antibody targeting NR1I2. Anti-NR1I2 Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-NR1I2 Polyclonal Antibody is a Rabbit antibody targeting NR1I2. Anti-NR1I2 Polyclonal Antibody can be used in WB,FCM.
AliasSteroid and xenobiotic receptor (SXR), PXR, Pregnane X receptor, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, Nuclear receptor subfamily 1 group I member 2, NR1I2
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Cow,Horse)
Verified Activity
1. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-PXR (TMAB-01265) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
2. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PXR antibody (TMAB-01265)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,FCM
Recommended Dose
WB: 1:500-2000; FCM: 2ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe human nuclear pregnane X receptor (PXR) activates cytochrome P450-3A expression in response to a wide variety of xenobiotics and plays a critical role in mediating dangerous drug-drug interactions. The human PXR is related to the mouse Pxr1, which they had cloned and shown to be activated by dexamethasone, pregnenolone 16-alpha-carbonitrile (PCN), and other compounds known to induce expression of the CYP3A1 gene, the predominant form of CYP3A in rat liver and intestine. Northern blot analysis detected most abundant expression in liver, colon, and small intestine; transcripts of 2.6, 4.3, and 5 kb were present in each of these tissues.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PXR
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Orphan Nuclear Receptors,Transcription Factors,Drug metabolism,Cancer,Hormones,Drug metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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