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Anti-NR1I2 Polyclonal Antibody is a Rabbit antibody targeting NR1I2. Anti-NR1I2 Polyclonal Antibody can be used in WB,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-NR1I2 Polyclonal Antibody is a Rabbit antibody targeting NR1I2. Anti-NR1I2 Polyclonal Antibody can be used in WB,FCM. |
Alias | Steroid and xenobiotic receptor (SXR), PXR, Pregnane X receptor, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, Nuclear receptor subfamily 1 group I member 2, NR1I2 |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Pig,Cow,Horse) |
Verified Activity | 1. Sample: Liver (Mouse) Lysate at 40 μg Primary: Anti-PXR (TMAB-01265) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa 2. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PXR antibody (TMAB-01265) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | WB,FCM |
Recommended Dose | WB: 1:500-2000; FCM: 2ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The human nuclear pregnane X receptor (PXR) activates cytochrome P450-3A expression in response to a wide variety of xenobiotics and plays a critical role in mediating dangerous drug-drug interactions. The human PXR is related to the mouse Pxr1, which they had cloned and shown to be activated by dexamethasone, pregnenolone 16-alpha-carbonitrile (PCN), and other compounds known to induce expression of the CYP3A1 gene, the predominant form of CYP3A in rat liver and intestine. Northern blot analysis detected most abundant expression in liver, colon, and small intestine; transcripts of 2.6, 4.3, and 5 kb were present in each of these tissues. |
Immunogen | KLH conjugated synthetic peptide: human PXR |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Orphan Nuclear Receptors,Transcription Factors,Drug metabolism,Cancer,Hormones,Drug metabolism |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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