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Anti-NOX1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01254

Anti-NOX1 Polyclonal Antibody is a Rabbit antibody targeting NOX1. Anti-NOX1 Polyclonal Antibody can be used in WB.

Anti-NOX1 Polyclonal Antibody

Anti-NOX1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01254
Anti-NOX1 Polyclonal Antibody is a Rabbit antibody targeting NOX1. Anti-NOX1 Polyclonal Antibody can be used in WB.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: WB

Reactivity: Human,Mouse (predicted:Rat,Dog,Cow,Horse,Rabbit)
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Cow,Horse,Rabbit)
Verified Activity
1. Sample: Testis (Mouse) Lysate at 40 μg
Primary: Anti-Nox1 (TMAB-01254) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 62 kDa
Observed band size: 57 kDa
2. Sample:
Testis (Mouse) Lysate at 40 μg
Uterus (Mouse) Lysate at 40 μg
DU145 (Human) Cell Lysate at 30 μg
Primary: Anti-Nox1 (TMAB-01254) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa
3. Sample:
Heart (Mouse) Lysate at 40 μg
Primary: Anti-Nox1 (TMAB-01254) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell projection, invadopodium membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Nox1
Antigen Species
Human
Gene ID
27035
Uniprot ID
Biology Area
Oxidative Stress,DNA Damage Response,Cytotoxic Cells,Nucleotide metabolism,Oxidative stress,Channels
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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