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Anti-NOX1 Polyclonal Antibody is a Rabbit antibody targeting NOX1. Anti-NOX1 Polyclonal Antibody can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: WB Reactivity: Human,Mouse (predicted:Rat,Dog,Cow,Horse,Rabbit) |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Dog,Cow,Horse,Rabbit) |
Verified Activity | 1. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-Nox1 (TMAB-01254) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 62 kDa Observed band size: 57 kDa 2. Sample: Testis (Mouse) Lysate at 40 μg Uterus (Mouse) Lysate at 40 μg DU145 (Human) Cell Lysate at 30 μg Primary: Anti-Nox1 (TMAB-01254) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 60 kDa 3. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-Nox1 (TMAB-01254) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa |
Application | WB |
Recommended Dose | WB: 1:500-2000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell projection, invadopodium membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation. |
Immunogen | KLH conjugated synthetic peptide: human Nox1 |
Antigen Species | Human |
Gene ID | 27035 |
Uniprot ID | |
Biology Area | Oxidative Stress,DNA Damage Response,Cytotoxic Cells,Nucleotide metabolism,Oxidative stress,Channels |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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