Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF

Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep)

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep)

1. U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (TMAB-01237) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Human U-87 MG cell Lysates
Lane 4: Human SH-SY5Y cell Lysates
Lane 5: Human U-2 OS cell Lysates
Primary: Anti-NGFB (TMAB-01237) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/32 kDa
Observed band size: 16/35 kDa
3. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Skin (Mouse) Lysate at 40 μg
Lane 3: Thyroid gland (Mouse) Lysate at 40 μg
Primary: Anti-NGFB (TMAB-01237) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32/13 kDa
Observed band size: 35/13 kDa
4. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NGFB Polyclonal Antibody, Unconjugated (TMAB-01237) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB,IHC-P,IHC-Fr,ICC/IF,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

Recombinded human NGF beta full length protein

Human

4803

Neurotrophins,Neuroregeneration