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Anti-NGF Polyclonal Antibody

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Catalog No. TMAB-01236
Alias β-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5, Beta-NGF

Anti-NGF Polyclonal Antibody is a Rabbit antibody targeting NGF. Anti-NGF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-NGF Polyclonal Antibody

Anti-NGF Polyclonal Antibody

😃Good
Catalog No. TMAB-01236Alias β-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5, Beta-NGF
Anti-NGF Polyclonal Antibody is a Rabbit antibody targeting NGF. Anti-NGF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-NGF Polyclonal Antibody is a Rabbit antibody targeting NGF. Anti-NGF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Aliasβ-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5, Beta-NGF
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NGF-beta Polyclonal Antibody, Unconjugated (TMAB-01236) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (TMAB-01236) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
3. Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Human U-87 MG cell Lysates
Lane 4: Human SH-SY5Y cell Lysates
Lane 5: Human U-2 OS cell Lysates
Primary: Anti-NGFB (TMAB-01236) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/32 kDa
Observed band size: 13/32 kDa
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NGF beta
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Neurotrophins,Neuroregeneration
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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