Anti-NCX1 Polyclonal Antibody is a Rabbit antibody targeting NCX1. Anti-NCX1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat,GuineaPig (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep)

1. Sample:
293t (Human) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
Panc-1 (Human) Cell Lysate at 30 μg
Primary: Anti-NCX1 (TMAB-01207) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 118 kDa
2. Sample:
HL-60 (Human) Cell Lysate at 30 μg
K562 (Human) Cell Lysate at 30 μg
Primary: Anti-NCX1 (TMAB-01207) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 118 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NCX1) Polyclonal Antibody, Unconjugated (TMAB-01207) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Sample:
Lane 1: U251 (Human) Cell Lysate at 30 μg
Lane 2: A431 (Human) Cell Lysate at 30 μg
Lane 3: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-NCX1 (TMAB-01207) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kDa
Observed band size: 120 kDa
5. Blank control: U937. Primary Antibody (green line): Rabbit Anti-NCX1 antibody (TMAB-01207)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
6. U-937 cells were fixed with 4% PFA for 10 min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL2 Antibody (TMAB-01207) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2ug/test

Polyclonal

KLH conjugated synthetic peptide: human NCX1

Human

Response to hypoxia,Inotropics,Hypoxia,Calcium Channels