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Anti-N Cadherin Antibody (2D207)

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Catalog No. TMAC-02739
Alias NCAD, CDw325, CDHN, CD325, cadherin 2, type 1, N-cadherin (neuronal)

Anti-N Cadherin Antibody (2D207) is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody (2D207) can be used in WB,ICC/IF,IHC,FCM.

Anti-N Cadherin Antibody (2D207)

Anti-N Cadherin Antibody (2D207)

😃Good
Catalog No. TMAC-02739Alias NCAD, CDw325, CDHN, CD325, cadherin 2, type 1, N-cadherin (neuronal)
Anti-N Cadherin Antibody (2D207) is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody (2D207) can be used in WB,ICC/IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4967-10 days
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Product Introduction

Bioactivity
Description
Anti-N Cadherin Antibody (2D207) is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody (2D207) can be used in WB,ICC/IF,IHC,FCM.
SynonymsNCAD, CDw325, CDHN, CD325, cadherin 2, type 1, N-cadherin (neuronal)
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of N Cadherin on different lysates using anti-N Cadherin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: Mouse brain.
2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-N Cadherin antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse embryo tissue using anti-N Cadherin antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-N Cadherin antibody. Counter stained with hematoxylin.
5. ICC staining N Cadherin in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining N Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining N Cadherin in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining N Cadherin in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of Hela cells with N Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundCadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy-terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 120/140 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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