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Anti-MTLC Polyclonal Antibody

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Catalog No. TMAB-01175
Alias MYCT1, MYCT 1 protein, Myc target protein 1, Myc target in myeloid cells protein 1, Myc target 1, Mt-Mc1, MTMC1, MTLC, MGC156310, MGC156309, FLJ21269, AI642973, AI225941, 1110020B04Rik

Anti-MTLC Polyclonal Antibody is a Rabbit antibody targeting MTLC. Anti-MTLC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-MTLC Polyclonal Antibody

Anti-MTLC Polyclonal Antibody

😃Good
Catalog No. TMAB-01175Alias MYCT1, MYCT 1 protein, Myc target protein 1, Myc target in myeloid cells protein 1, Myc target 1, Mt-Mc1, MTMC1, MTLC, MGC156310, MGC156309, FLJ21269, AI642973, AI225941, 1110020B04Rik
Anti-MTLC Polyclonal Antibody is a Rabbit antibody targeting MTLC. Anti-MTLC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-MTLC Polyclonal Antibody is a Rabbit antibody targeting MTLC. Anti-MTLC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
SynonymsMYCT1, MYCT 1 protein, Myc target protein 1, Myc target in myeloid cells protein 1, Myc target 1, Mt-Mc1, MTMC1, MTLC, MGC156310, MGC156309, FLJ21269, AI642973, AI225941, 1110020B04Rik
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog)
Verified Activity
1. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-MTLC (TMAB-01175) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 31 kDa
2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MTLC Polyclonal Antibody, Unconjugated (TMAB-01175) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Note=Expressed in nuclei of hepatocellular carcinoma cell line Bel7402 cells.
Tissue SpecificityDown-regulated in gastric cancer tissues.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundMTLC is a 235 amino acid nuclear protein that is thought to regulate certain myc target genes. Downregulated in gastric cancer tissues, MTLC is expressed in the hepatocellular carcinoma cell line, Bel7402. Overexpression of MTLC is thought to influence tumorigenic conversion, promotion of apoptosis genomic instability and inhibition of hematopoietic differentiation. The gene encoding MTLC maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MTLC
Antigen Species
Human
Gene Name
MYCT1
Gene ID
Protein Name
Myc target protein 1
Uniprot ID
Biology Area
Transcription Factors
Function
May regulate certain MYC target genes, MYC seems to be a direct upstream transcriptional activator. Does not seem to significantly affect growth cell capacity. Overexpression seems to mediate many of the known phenotypic features associated with MYC, including promotion of apoptosis, alteration of morphology, enhancement of anchorage-independent growth, tumorigenic conversion, promotion of genomic instability, and inhibition of hematopoietic differentiation.
Chemical Properties
Molecular WeightTheoretical: 27 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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