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Anti-MTLC Polyclonal Antibody
😃Good
Catalog No. TMAB-01175
Anti-MTLC Polyclonal Antibody is a Rabbit antibody targeting MTLC. Anti-MTLC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-MTLC Polyclonal Antibody
Anti-MTLC Polyclonal Antibody
😃Good
Catalog No. TMAB-01175
Anti-MTLC Polyclonal Antibody is a Rabbit antibody targeting MTLC. Anti-MTLC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-MTLC Polyclonal Antibody is a Rabbit antibody targeting MTLC. Anti-MTLC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog) |
| Verified Activity | 1. Sample: Brain (Mouse) Lysate at 40 μg Primary: Anti-MTLC (TMAB-01175) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 27 kDa Observed band size: 31 kDa 2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-MTLC Polyclonal Antibody, Unconjugated (TMAB-01175) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Note=Expressed in nuclei of hepatocellular carcinoma cell line Bel7402 cells. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | MTLC is a 235 amino acid nuclear protein that is thought to regulate certain myc target genes. Downregulated in gastric cancer tissues, MTLC is expressed in the hepatocellular carcinoma cell line, Bel7402. Overexpression of MTLC is thought to influence tumorigenic conversion, promotion of apoptosis genomic instability and inhibition of hematopoietic differentiation. The gene encoding MTLC maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6. |
| Immunogen | KLH conjugated synthetic peptide: human MTLC |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Transcription Factors |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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