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Anti-MTCO2 Antibody (5B251)
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Catalog No. TMAC-02671
Alias MT-CO2, MTCO2, MTCO 2, MT CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, Cytochrome c oxidase II, COXII, COX2_HUMAN, COX2, COII
Anti-MTCO2 Antibody (5B251) is a Rabbit antibody targeting MTCO2. Anti-MTCO2 Antibody (5B251) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-MTCO2 Antibody (5B251)
Anti-MTCO2 Antibody (5B251)
😃Good
Catalog No. TMAC-02671Alias MT-CO2, MTCO2, MTCO 2, MT CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, Cytochrome c oxidase II, COXII, COX2_HUMAN, COX2, COII
Anti-MTCO2 Antibody (5B251) is a Rabbit antibody targeting MTCO2. Anti-MTCO2 Antibody (5B251) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-MTCO2 Antibody (5B251) is a Rabbit antibody targeting MTCO2. Anti-MTCO2 Antibody (5B251) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | MT-CO2, MTCO2, MTCO 2, MT CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, Cytochrome c oxidase II, COXII, COX2_HUMAN, COX2, COII |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of MTCO2 on different lysates using anti-MTCO2 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7, Lane 2: THP-1, Lane 3: Hela. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin. 3. ICC staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cytochrome c oxidase subunit II (COX2), also designated COII, MTCO2 or oxidative phosphorylation (OxPhos) complex IV, subunit II, is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electrochemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded. Defects in COX2 are associated with tumor formation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 25 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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