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Anti-MMP-13 Polyclonal Antibody
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Catalog No. TMAB-01151
Alias MMP13, Matrix metalloproteinase-13 (MMP-13), Collagenase 3
Anti-MMP-13 Polyclonal Antibody is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-MMP-13 Polyclonal Antibody
Anti-MMP-13 Polyclonal Antibody
😃Good
Catalog No. TMAB-01151Alias MMP13, Matrix metalloproteinase-13 (MMP-13), Collagenase 3
Anti-MMP-13 Polyclonal Antibody is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-MMP-13 Polyclonal Antibody is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | MMP13, Matrix metalloproteinase-13 (MMP-13), Collagenase 3 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Rabbit) |
| Verified Activity | 1. Tissue/cell: rat articular cartilage; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated (TMAB-01151) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated (TMAB-01151) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-MMP-13 Polyclonal Antibody, Unconjugated (TMAB-01151) at 1:200 followed by conjugation to the secondary antibody and DAb staining. 4. Sample: Ovary (Rat) lysate at 40 μg Primary: Anti-MMP13 (TMAB-01151) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa 5. MG63 (Human) lysate at 30 μg U 2Os (Human) lysate at 30 μg MDA-MB-231 (Human) lysate at 30 μg Primary: Anti-MMP13 (TMAB-01151) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 60 kDa 6. Sample: Embryo (Mouse) Lysate at 40 μg Primary: Anti-MMP13 (TMAB-01151) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix (Probable). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human MMP13 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | MMPs,MMPs,MMPs,MMP,MMPs,Tumor biomarkers ELISA kits,MMP |
| Molecular Weight | Theoretical: 52 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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