Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-MMP-13 Polyclonal Antibody

😃Good
Catalog No. TMAB-01151

Anti-MMP-13 Polyclonal Antibody is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-MMP-13 Polyclonal Antibody

Anti-MMP-13 Polyclonal Antibody

😃Good
Catalog No. TMAB-01151
Anti-MMP-13 Polyclonal Antibody is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$528 7-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-MMP-13 Polyclonal Antibody is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Rabbit)
Verified Activity
1. Tissue/cell: rat articular cartilage; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated (TMAB-01151) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated (TMAB-01151) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse bone labeled with Anti-MMP-13 Polyclonal Antibody, Unconjugated (TMAB-01151) at 1:200 followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
Ovary (Rat) lysate at 40 μg
Primary: Anti-MMP13 (TMAB-01151) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
5. MG63 (Human) lysate at 30 μg
U 2Os (Human) lysate at 30 μg
MDA-MB-231 (Human) lysate at 30 μg
Primary: Anti-MMP13 (TMAB-01151) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 60 kDa
6. Sample:
Embryo (Mouse) Lysate at 40 μg
Primary: Anti-MMP13 (TMAB-01151) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space, extracellular matrix (Probable).
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MMP13
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
MMPs,MMPs,MMPs,MMP,MMPs,Tumor biomarkers ELISA kits,MMP
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-MMP-13 Polyclonal Antibody | purchase Anti-MMP-13 Polyclonal Antibody | Anti-MMP-13 Polyclonal Antibody cost | order Anti-MMP-13 Polyclonal Antibody