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Anti-MKI67 Antibody (9T614)
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Catalog No. TMAB-01143
Alias Proliferation marker protein Ki-67, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67;Antigen Ki67)
Anti-MKI67 Antibody (9T614) is a Mouse antibody targeting MKI67. Anti-MKI67 Antibody (9T614) can be used in FCM.
Anti-MKI67 Antibody (9T614)
Anti-MKI67 Antibody (9T614)
😃Good
Catalog No. TMAB-01143Alias Proliferation marker protein Ki-67, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67;Antigen Ki67)
Anti-MKI67 Antibody (9T614) is a Mouse antibody targeting MKI67. Anti-MKI67 Antibody (9T614) can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-MKI67 Antibody (9T614) is a Mouse antibody targeting MKI67. Anti-MKI67 Antibody (9T614) can be used in FCM. |
| Alias | Proliferation marker protein Ki-67, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67;Antigen Ki67) |
| Ig Type | IgG |
| Clone | 9T614 |
| Reactivity | Human |
| Verified Activity | Blank control: Hela. Primary Antibody (green line): Mouse Anti-Ki-67 antibody (TMAB-01143) Dilution: 1 μg/Test; Secondary Antibody: Goat anti-mouse IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | |
| Recommended Dose | FCM: 1ug/Test |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
| Immunogen | KLH conjugated synthetic peptide: human Ki-67 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cell division,Tumor biomarkers,Markers,Soma marker,Neurogenesis,Replication |
| Molecular Weight | Theoretical: 358 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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