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Anti-MIF Antibody (9F31)
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Catalog No. TMAC-02585
Alias MMIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), GLIF, GIF
Anti-MIF Antibody (9F31) is a Rabbit antibody targeting MIF. Anti-MIF Antibody (9F31) can be used in WB,IP,FCM.
Anti-MIF Antibody (9F31)
Anti-MIF Antibody (9F31)
😃Good
Catalog No. TMAC-02585Alias MMIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), GLIF, GIF
Anti-MIF Antibody (9F31) is a Rabbit antibody targeting MIF. Anti-MIF Antibody (9F31) can be used in WB,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-MIF Antibody (9F31) is a Rabbit antibody targeting MIF. Anti-MIF Antibody (9F31) can be used in WB,IP,FCM. |
| Synonyms | MMIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), GLIF, GIF |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution. Positive control: Line 1: Mouse brain, Line 2: Jurkat, Line 3: Hela. 2. Flow cytometric analysis of THP-1 cells with MIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor α (TNFα), interleukin-1 (IL-1) and interferon-γ (IFN-γ). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 12 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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