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Anti-MBD3 Antibody (8O286)
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Catalog No. TMAC-02484
Alias Methyl-CpG-binding protein MBD3, Methyl-CpG-binding domain protein 3, Methyl CpG binding protein MBD3, Methyl CpG binding domain protein 3, MBD3_HUMAN, MBD3: methyl CpG binding domain protein 3, Mbd3, MBD 3, AU019209, AI181826
Anti-MBD3 Antibody (8O286) is a Rabbit antibody targeting MBD3. Anti-MBD3 Antibody (8O286) can be used in WB,ICC,IF,IHC,FCM,IP.
Anti-MBD3 Antibody (8O286)
Anti-MBD3 Antibody (8O286)
😃Good
Catalog No. TMAC-02484Alias Methyl-CpG-binding protein MBD3, Methyl-CpG-binding domain protein 3, Methyl CpG binding protein MBD3, Methyl CpG binding domain protein 3, MBD3_HUMAN, MBD3: methyl CpG binding domain protein 3, Mbd3, MBD 3, AU019209, AI181826
Anti-MBD3 Antibody (8O286) is a Rabbit antibody targeting MBD3. Anti-MBD3 Antibody (8O286) can be used in WB,ICC,IF,IHC,FCM,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-MBD3 Antibody (8O286) is a Rabbit antibody targeting MBD3. Anti-MBD3 Antibody (8O286) can be used in WB,ICC,IF,IHC,FCM,IP. |
| Synonyms | Methyl-CpG-binding protein MBD3, Methyl-CpG-binding domain protein 3, Methyl CpG binding protein MBD3, Methyl CpG binding domain protein 3, MBD3_HUMAN, MBD3: methyl CpG binding domain protein 3, Mbd3, MBD 3, AU019209, AI181826 |
| Ig Type | IgG |
| Clone | 8O286 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of MBD3 on U937 cell lysate using anti-MBD3 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-MBD3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MBD3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MBD3 antibody. Counter stained with hematoxylin. 5. ICC staining MBD3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining MBD3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining MBD3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of SH-SY-5Y cells with MBD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5?? cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites, and they include methyl-CpG binding protein-1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 33 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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