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Anti-KRT19 Antibody (2L986) is a Mouse antibody targeting KRT19. Anti-KRT19 Antibody (2L986) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-KRT19 Antibody (2L986) is a Mouse antibody targeting KRT19. Anti-KRT19 Antibody (2L986) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
Alias | type I cytoskeletal 19, KRT19, Keratin-19 (K19), Keratin, type I cytoskeletal 19, Cytokeratin-19 (CK-19) |
Ig Type | IgG |
Clone | 2L986 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Cytokeratin 19) monoclonal Antibody, Unconjugated (TMAB-01038) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Paraformaldehyde-fixed, paraffin embedded (Human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Human esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (mouse bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Rectal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Rat Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 13. 25 μg total protein per Lane of various lysates probed with Cytokeratin 19 monoclonal antibody, unconjugated (TMAB-01038) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 14. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:500 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 15. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:500 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-1500; IHC-Fr: 1:100-1500; ICC/IF: 1:100; IF: 1:100-1500 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein G purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. |
Immunogen | KLH conjugated synthetic peptide: human Cytokeratin 19 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Cytokeratins |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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