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Anti-KRT19 Antibody (2L986)

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Catalog No. TMAB-01038
Alias type I cytoskeletal 19, KRT19, Keratin-19 (K19), Keratin, type I cytoskeletal 19, Cytokeratin-19 (CK-19)

Anti-KRT19 Antibody (2L986) is a Mouse antibody targeting KRT19. Anti-KRT19 Antibody (2L986) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-KRT19 Antibody (2L986)

Anti-KRT19 Antibody (2L986)

😃Good
Catalog No. TMAB-01038Alias type I cytoskeletal 19, KRT19, Keratin-19 (K19), Keratin, type I cytoskeletal 19, Cytokeratin-19 (CK-19)
Anti-KRT19 Antibody (2L986) is a Mouse antibody targeting KRT19. Anti-KRT19 Antibody (2L986) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-KRT19 Antibody (2L986) is a Mouse antibody targeting KRT19. Anti-KRT19 Antibody (2L986) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Aliastype I cytoskeletal 19, KRT19, Keratin-19 (K19), Keratin, type I cytoskeletal 19, Cytokeratin-19 (CK-19)
Ig Type
IgG
Clone
2L986
Reactivity
Human,Mouse,Rat
Verified Activity
1. MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Cytokeratin 19) monoclonal Antibody, Unconjugated (TMAB-01038) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Paraformaldehyde-fixed, paraffin embedded (Human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Human esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Rectal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (ascites of TMAB-01038) at 1:800 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Rat Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
13. 25 μg total protein per Lane of various lysates probed with Cytokeratin 19 monoclonal antibody, unconjugated (TMAB-01038) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
14. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:500 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
15. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (TMAB-01038) at 1:500 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-1500; IHC-Fr: 1:100-1500; ICC/IF: 1:100; IF: 1:100-1500
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Cytokeratin 19
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cytokeratins
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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