Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-KRT14 Antibody (2V710)
😃Good
Catalog No. TMAY-00070
Alias NFJ, K14, EBS4, EBS3, EBS1D, EBS1C, EBS1B, EBS1A, EBS1, CK14
Anti-KRT14 Antibody (2V710) is a Rabbit antibody targeting KRT14. Anti-KRT14 Antibody (2V710) can be used in WB,IHC-P,IP.
Anti-KRT14 Antibody (2V710)
Anti-KRT14 Antibody (2V710)
😃Good
Catalog No. TMAY-00070Alias NFJ, K14, EBS4, EBS3, EBS1D, EBS1C, EBS1B, EBS1A, EBS1, CK14
Anti-KRT14 Antibody (2V710) is a Rabbit antibody targeting KRT14. Anti-KRT14 Antibody (2V710) can be used in WB,IHC-P,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IHC-P, IP Reactivity: Human |
| Alias | NFJ, K14, EBS4, EBS3, EBS1D, EBS1C, EBS1B, EBS1A, EBS1, CK14 |
| Ig Type | Rabbit IgG |
| Clone | 2V710 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human CK14 in human skin with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human CK14 in human esophageal carcinoma with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). 3. KRT14 was immunoprecipitated using: -Lane A:0.5 mg A431 Whole Cell Lysate. -2 µL anti-KRT14 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-KRT14 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 52 kDa. -Observed band size: 52 kDa. 4. Anti-Cytokeratin14 rabbit monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:52 kDa. -Observed band size:52 kDa |
| Application | WB,IHC-P,IP |
| Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:10000-1:20000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, a synthetic peptide corresponding to the N-terminus of the human Cytokeratin 14. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Cytokeratin 14, also known as Keratin 14 and K14, is a member of the keratin family. Cytokeratin 14 is a type I keratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Cytokeratin 14 is mainly expressed in the basal layer. It is also strongly expressed in the outer root sheath of anagen follicles. Cytokeratin 14 and keratin 5 may have a role in the maintenance of cell proliferation potential in the basal layer of stratified epithelia, modulating phosphatidylinositol 3-kinase/Akt–mediated cell proliferation and/or Notch1-dependent cell differentiation. Cytokeratin 14 defect prevents it from working effectively with keratin 5 and interfering with the assembly of the keratin intermediate filament network. A disruption in this network makes keratinocytes fragile and prone to rupture. Minor trauma to the skin, such as rubbing or scratching, can cause these cells to break down, resulting in the formation of painful, fluid-filled blisters. Mutations in the K14 gene are also responsible for Naegeli-Franceschetti-Jadassohn syndrome and Dermatopathia Pigmentosa Reticularis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: N-terminus of the human Cytokeratin 14. |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-KRT14 Antibody (2V710) | purchase Anti-KRT14 Antibody (2V710) | Anti-KRT14 Antibody (2V710) cost | order Anti-KRT14 Antibody (2V710)
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.