Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-KDEL Antibody (8Y233)
😃Good
Catalog No. TMAC-02300
Alias Putative MAPK-activating protein PM23, Kdelr1, KDEL receptor 1, KDEL endoplasmic reticulum protein retention receptor 1, ERD21_HUMAN, ERD2.1, ER lumen protein retaining receptor 1
Anti-KDEL Antibody (8Y233) is a Rabbit antibody targeting KDEL. Anti-KDEL Antibody (8Y233) can be used in WB,ICC,IF,IHC,FCM.
Anti-KDEL Antibody (8Y233)
Anti-KDEL Antibody (8Y233)
😃Good
Catalog No. TMAC-02300Alias Putative MAPK-activating protein PM23, Kdelr1, KDEL receptor 1, KDEL endoplasmic reticulum protein retention receptor 1, ERD21_HUMAN, ERD2.1, ER lumen protein retaining receptor 1
Anti-KDEL Antibody (8Y233) is a Rabbit antibody targeting KDEL. Anti-KDEL Antibody (8Y233) can be used in WB,ICC,IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
Add to Cart
Add to Quotation
Bulk & Custom
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View MoreResource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-KDEL Antibody (8Y233) is a Rabbit antibody targeting KDEL. Anti-KDEL Antibody (8Y233) can be used in WB,ICC,IF,IHC,FCM. |
| Synonyms | Putative MAPK-activating protein PM23, Kdelr1, KDEL receptor 1, KDEL endoplasmic reticulum protein retention receptor 1, ERD21_HUMAN, ERD2.1, ER lumen protein retaining receptor 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1,000 dilution. Positive control: Lane 1: Rat testis tissue, Lane 2: Human placenta tissue, Lane 3: Mouse testis tissue, Lane 4: 293. 2. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KDEL antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-KDEL antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-KDEL antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-KDEL antibody. Counter stained with hematoxylin. 6. ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining KDEL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining KDEL in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of HepG2 cells with KDEL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:100-400; ICC: 1:400-800; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Soluble proteins in the endoplasmic reticulum (ER) contain a specific carboxy terminal sequence KDEL (Lys-Asp-Glu-Leu), and include the coat proteins required for vesicle budding from the ER, proteins that form retrograde vesicles on post-ER compartments, and integral membrane proteins that target vesicles to their correct destination. The retention of these soluble proteins in the ER depends on the interaction of the KDEL sequence with the corresponding KDEL receptor, also designated ERD2, in the Golgi apparatus. When KDEL proteins reach the Golgi complex, they are recognized by the KDEL receptor and transported retrograde in COPI-coated vesicles back to the ER. The small GTPase ADP-ribosylation factor 1 (ARF1), a regulator of vesicle transport, interacts with the KDEL receptor. Subsequently, this interaction allows the KDEL receptor to recruit a GTPase-activating protein (GAP) from the cytosol to membranes, which inactivates ARF1. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 24/17 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Sci Citations
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-KDEL Antibody (8Y233) | purchase Anti-KDEL Antibody (8Y233) | Anti-KDEL Antibody (8Y233) cost | order Anti-KDEL Antibody (8Y233) | Anti-KDEL Antibody (8Y233) molecular weight
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.