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Anti-JARID2 Antibody (7C209)
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Catalog No. TMAC-02279
Alias Protein Jumonji, Jumonji/ARID domain-containing protein 2, Jumonji/ARID domain containing protein 2, Jumonji protein, Jumonji like protein, Jumonji homolog, Jumonji AT rich interactive domain 2, JMJ, JARID2, JARID 2, JARD2_HUMAN, JARD2
Anti-JARID2 Antibody (7C209) is a Mouse antibody targeting JARID2. Anti-JARID2 Antibody (7C209) can be used in WB,ICC,FCM.
Anti-JARID2 Antibody (7C209)
Anti-JARID2 Antibody (7C209)
😃Good
Catalog No. TMAC-02279Alias Protein Jumonji, Jumonji/ARID domain-containing protein 2, Jumonji/ARID domain containing protein 2, Jumonji protein, Jumonji like protein, Jumonji homolog, Jumonji AT rich interactive domain 2, JMJ, JARID2, JARID 2, JARD2_HUMAN, JARD2
Anti-JARID2 Antibody (7C209) is a Mouse antibody targeting JARID2. Anti-JARID2 Antibody (7C209) can be used in WB,ICC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $428 | 7-10 days |
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| Description | Anti-JARID2 Antibody (7C209) is a Mouse antibody targeting JARID2. Anti-JARID2 Antibody (7C209) can be used in WB,ICC,FCM. |
| Synonyms | Protein Jumonji, Jumonji/ARID domain-containing protein 2, Jumonji/ARID domain containing protein 2, Jumonji protein, Jumonji like protein, Jumonji homolog, Jumonji AT rich interactive domain 2, JMJ, JARID2, JARID 2, JARD2_HUMAN, JARD2 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of JARID2 on human JARID2 recombinant protein using anti-JARID2 antibody at 1/1,000 dilution. 2. Western blot analysis of JARID2 on HEK293 (1) and JARID2-hIgGFc transfected HEK293 (2) cell lysate using anti-JARID2 antibody at 1/1,000 dilution. 3. ICC staining JARID2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Hela cells with JARID2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC: 1:50-200; FCM: 1:100-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 139 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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