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Anti-IRE1 Polyclonal Antibody
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Catalog No. TMAB-00982
Alias IRE1P, IRE1a, IRE1, hIRE1p, endoplasmic reticulum to nucleus signaling 1
Anti-IRE1 Polyclonal Antibody is a Rabbit antibody targeting IRE1. Anti-IRE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-IRE1 Polyclonal Antibody
Anti-IRE1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00982Alias IRE1P, IRE1a, IRE1, hIRE1p, endoplasmic reticulum to nucleus signaling 1
Anti-IRE1 Polyclonal Antibody is a Rabbit antibody targeting IRE1. Anti-IRE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-IRE1 Polyclonal Antibody is a Rabbit antibody targeting IRE1. Anti-IRE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | IRE1P, IRE1a, IRE1, hIRE1p, endoplasmic reticulum to nucleus signaling 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Pig (predicted:Rat) |
| Verified Activity | 1. Sample: Pig Primary: Anti-IRE1 (TMAB-00982) at 1/500 dilution 2. Sample: Pancreas (Mouse) Lysate at 40 μg Primary: Anti-IRE1 (TMAB-00982) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 105 kDa Observed band size:105 kDa 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRE1) Polyclonal Antibody, Unconjugated (TMAB-00982) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | ERN2, endoplasmic reticulum to nucleus signaling 2, induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. It is pro-apoptotic and appears to play no role in the unfolded-protein response, unlike closely related proteins. |
| Immunogen | KLH conjugated synthetic peptide: human IRE1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Other Kinases |
| Molecular Weight | Theoretical: 105 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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