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Anti-IRE1 Polyclonal Antibody

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Catalog No. TMAB-00982
Alias IRE1P, IRE1a, IRE1, hIRE1p, endoplasmic reticulum to nucleus signaling 1

Anti-IRE1 Polyclonal Antibody is a Rabbit antibody targeting IRE1. Anti-IRE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-IRE1 Polyclonal Antibody

Anti-IRE1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00982Alias IRE1P, IRE1a, IRE1, hIRE1p, endoplasmic reticulum to nucleus signaling 1
Anti-IRE1 Polyclonal Antibody is a Rabbit antibody targeting IRE1. Anti-IRE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-IRE1 Polyclonal Antibody is a Rabbit antibody targeting IRE1. Anti-IRE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasIRE1P, IRE1a, IRE1, hIRE1p, endoplasmic reticulum to nucleus signaling 1
Ig Type
IgG
Reactivity
Human,Mouse,Pig (predicted:Rat)
Verified Activity
1. Sample: Pig
Primary: Anti-IRE1 (TMAB-00982) at 1/500 dilution
2. Sample:
Pancreas (Mouse) Lysate at 40 μg
Primary: Anti-IRE1 (TMAB-00982) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 105 kDa
Observed band size:105 kDa
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRE1) Polyclonal Antibody, Unconjugated (TMAB-00982) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundERN2, endoplasmic reticulum to nucleus signaling 2, induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. It is pro-apoptotic and appears to play no role in the unfolded-protein response, unlike closely related proteins.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human IRE1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Other Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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