Anti-IRE1 Polyclonal Antibody is a Rabbit antibody targeting IRE1. Anti-IRE1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

IgG

Mouse,Pig (predicted:Human,Rat)

1. Sample: Pig
Primary: Anti-IRE1 (TMAB-00982) at 1/500 dilution
2. Sample:
Pancreas (Mouse) Lysate at 40 μg
Primary: Anti-IRE1 (TMAB-00982) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 105 kDa
Observed band size:105 kDa
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRE1) Polyclonal Antibody, Unconjugated (TMAB-00982) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human IRE1

Human

ERN1

Serine/threonine-protein kinase/endoribonuclease IRE1

Other Kinases

Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins.