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Anti-HSP70 Antibody (7N158)
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Catalog No. TMAC-02046
Alias HSP70, Heat shock 70 kDa protein
Anti-HSP70 Antibody (7N158) is a Rabbit antibody targeting HSP70. Anti-HSP70 Antibody (7N158) can be used in WB,ICC/IF,IHC,FCM.
Anti-HSP70 Antibody (7N158)
Anti-HSP70 Antibody (7N158)
😃Good
Catalog No. TMAC-02046Alias HSP70, Heat shock 70 kDa protein
Anti-HSP70 Antibody (7N158) is a Rabbit antibody targeting HSP70. Anti-HSP70 Antibody (7N158) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-HSP70 Antibody (7N158) is a Rabbit antibody targeting HSP70. Anti-HSP70 Antibody (7N158) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | HSP70, Heat shock 70 kDa protein |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Hsp70 on different cell lysates using anti-Hsp70 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7, Lane 2: HCT116. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsp70 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Hsp70 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hsp70 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Hsp70 antibody. Counter stained with hematoxylin. 6. ICC staining Hsp70 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Hsp70 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Hsp70 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:10-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressedheat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Molecular Weight | Theoretical: 70 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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