Anti-HOXD8 Polyclonal Antibody is a Rabbit antibody targeting HOXD8. Anti-HOXD8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Pig,Cow,Horse,Sheep)

1. Protein: muscle (mouse) lysate at 40 μg;
Primary: rabbit Anti-HOXD8 (TMAB-07263) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000;
Predicted band size: 32 kD
Observed band size: 34 kD
2. Tissue/cell: Human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-HOXC8 Polyclonal Antibody, Unconjugated (TMAB-07263) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HOXD8

Human

HOXD8

Homeobox protein Hox-D8

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.