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Anti-HIST1H1C Antibody (3S922) is a Rabbit antibody targeting HIST1H1C. Anti-HIST1H1C Antibody (3S922) can be used in WB,ICC,IF,IHC,ChIP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-HIST1H1C Antibody (3S922) is a Rabbit antibody targeting HIST1H1C. Anti-HIST1H1C Antibody (3S922) can be used in WB,ICC,IF,IHC,ChIP. |
Synonyms | Histone H1s-1, Histone H1d, Histone H1c, Histone H1.2, H1F2 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution. Positive control: Lane 1: Hela, Lane 2: 293, Lane 3: MCF-7. 2. Western blot analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin. 6. ICC staining Histone H1.2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Histone H1.2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Histone H1.2 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 29 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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