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Anti-HGPRT Antibody (2V553)
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Catalog No. TMAC-02013
Alias Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1, HPRT, HGPRTase, HGPRT
Anti-HGPRT Antibody (2V553) is a Rabbit antibody targeting HGPRT. Anti-HGPRT Antibody (2V553) can be used in WB,IHC,IP.
Anti-HGPRT Antibody (2V553)
Anti-HGPRT Antibody (2V553)
😃Good
Catalog No. TMAC-02013Alias Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1, HPRT, HGPRTase, HGPRT
Anti-HGPRT Antibody (2V553) is a Rabbit antibody targeting HGPRT. Anti-HGPRT Antibody (2V553) can be used in WB,IHC,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-HGPRT Antibody (2V553) is a Rabbit antibody targeting HGPRT. Anti-HGPRT Antibody (2V553) can be used in WB,IHC,IP. |
| Synonyms | Hypoxanthine-Guanine Phosphoribosyltransferase, HPRT1, HPRT, HGPRTase, HGPRT |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,zebrafish |
| Verified Activity | 1. Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution. Positive control:Lane 1: Hela, Lane 2: A431, Lane 3: MCF-7, Lane 4: 293, Lane 5: Rat kidney, Lane 6: Rat brain, Lane 7: Mouse testis, Lane 8: Mouse colon. 2. Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution. 3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; IP: 1:10-50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 25 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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