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Anti-HDAC6 Polyclonal Antibody
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Catalog No. TMAB-00841
Anti-HDAC6 Polyclonal Antibody is a Rabbit antibody targeting HDAC6. Anti-HDAC6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HDAC6 Polyclonal Antibody
Anti-HDAC6 Polyclonal Antibody
😃Good
Catalog No. TMAB-00841
Anti-HDAC6 Polyclonal Antibody is a Rabbit antibody targeting HDAC6. Anti-HDAC6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-HDAC6 Polyclonal Antibody is a Rabbit antibody targeting HDAC6. Anti-HDAC6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-HDAC6 (TMAB-00841) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 134 kDa Observed band size: 134 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HDAC6) Polyclonal Antibody, Unconjugated (TMAB-00841) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Note=It is mainly cytoplasmic, where it is associated with microtubules. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | HDAC6 is a member of the class II mammalian histone deacetylases. Human HDAC6 is composed of 1215 amino acid residues. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. |
| Immunogen | KLH conjugated synthetic peptide: human HDAC6 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Acetylation,Class II / Hda1 Class,HDACs |
| Molecular Weight | Theoretical: 134 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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