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Anti-HDAC3 Polyclonal Antibody is a Rabbit antibody targeting HDAC3. Anti-HDAC3 Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-HDAC3 Polyclonal Antibody is a Rabbit antibody targeting HDAC3. Anti-HDAC3 Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB. |
Synonyms | SMAP45, RPD3-2, Protein deacylase HDAC3, Protein deacetylase HDAC3, Histone deacetylase 3, HDAC3, HD3 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Horse) |
Verified Activity | 1. issue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HDAC3/HD3 Polyclonal Antibody, Unconjugated (TMAB-00840) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. 2. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HDAC3/HD3 Polyclonal Antibody, Unconjugated (TMAB-00840) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated at 37°C for 20 min; Incubation: Anti-HDAC3/HD3 Polyclonal Antibody, Unconjugated (TMAB-00840) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HDAC3) Polyclonal Antibody, Unconjugated (TMAB-00840) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Sample: Stomach (Mouse) Lysate at 40 μg Primary: Anti-HDAC3 (TMAB-00840) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa 6. Sample: Uterus (Mouse) Lysate at 40 μg Ovary (Mouse) Lysate at 40 μg Primary: Anti-HDAC3 (TMAB-00840) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 50 kDa 7. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-HDAC3/HD3 (TMAB-00840) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa |
Application | |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. |
Tissue Specificity | Widely expressed. |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Concentration | 1 mg/mL |
Immunogen | KLH conjugated synthetic peptide: human HDAC3 |
Antigen Species | Human |
Gene Name | HDAC3 |
Gene ID | |
Protein Name | Histone deacetylase 3 |
Uniprot ID | |
Biology Area | Metabolism of lipids and lipoproteins,Tumor suppressors,Hypertrophy,Acetylation,Class I,Lipid metabolism,Cancer,HDACs |
Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. |
Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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