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Anti-HDAC1 Polyclonal Antibody

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Catalog No. TMAB-00839

Anti-HDAC1 Polyclonal Antibody is a Rabbit antibody targeting HDAC1. Anti-HDAC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-HDAC1 Polyclonal Antibody

Anti-HDAC1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00839
Anti-HDAC1 Polyclonal Antibody is a Rabbit antibody targeting HDAC1. Anti-HDAC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
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Product Introduction

Bioactivity
Description
Anti-HDAC1 Polyclonal Antibody is a Rabbit antibody targeting HDAC1. Anti-HDAC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (KO Validated) hDAC1 polyclonal Antibody, Unconjugated (TMAB-00839) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (KO Validated) hDAC1 polyclonal Antibody, Unconjugated (TMAB-00839) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
3. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HDAC1) Polyclonal Antibody, Unconjugated (TMAB-00839) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HDAC1) Polyclonal Antibody, Unconjugated (TMAB-00839) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HDAC1) Polyclonal Antibody, Unconjugated (TMAB-00839) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: Molt-4 (Human) Cell Lysate at 30 μg
Lane 3: U251 (Human) Cell Lysate at 30 μg
Lane 4: MCF-7 (Human) Cell Lysate at 30 μg
Primary:
Anti-HDAC1 (TMAB-00839) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:100; IF: 1:50-100
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundHistone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
Recombinant Protein: human HDAC1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Hypertrophy,Acetylation,Class I,HDAC1,HDACs
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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