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Anti-GOLGA7 Polyclonal Antibody

Catalog No. TMAB-06631 Copy Product Info
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Anti-GOLGA7 Polyclonal Antibody is a Rabbit antibody targeting GOLGA7. Anti-GOLGA7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-GOLGA7 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-06631

Anti-GOLGA7 Polyclonal Antibody is a Rabbit antibody targeting GOLGA7. Anti-GOLGA7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-GOLGA7 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GOLGA7 Polyclonal Antibody is a Rabbit antibody targeting GOLGA7. Anti-GOLGA7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Sheep)
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GOLGA7) Polyclonal Antibody, Unconjugated (TMAB-06631) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus membrane; Lipid-anchor
Tissue SpecificityExpressed in all tissues except colon and thymus.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundGOLGA7 is a multi-pass membrane protein belonging to the Erf4 family of proteins. It is the functional ortholog of the yeast Erf4 protein. Localizing to the Golgi apparatus, GOLGA7 is a widely expressed protein but its expression is absent from colon and thymus tissues. GOLGA7 is palmitoylated on two cysteine residues, and this palmitoylation is required for its interaction with golgin 160 and its Golgi-localization. GOLGA7 also forms a complex with ZDHHC9 and, together, these proteins function as a Ras palmitoyltransferase (Ras PAT) which is required for palmitoylation of H-Ras and N-Ras proteins. The palmitoylation of Ras proteins is essential for the trafficking of Ras proteins from the Golgi to the plasma membrane, thus implicating GOLGA7 in protein transport from the Golgi to the cell surface.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GOLGA7
Antigen Species
Human
Gene Name
GOLGA7
Gene ID
Protein Name
Golgin subfamily A member 7
Function
GOLGA7 (Golgi autoantigen, golgin subfamily a, 7) is a member of the golgin family and may be involved in protein transport from the Golgi to the cell surface. GOLGA7 forms a protein complex with ZDHHC9. This ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for H-RAS and N-RAS.
Chemical Properties
Molecular WeightTheoretical: 16 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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