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Anti-GAP43 Antibody (6G327)
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Catalog No. TMAC-01612
Alias PP46, growth associated protein 43, B-50
Anti-GAP43 Antibody (6G327) is a Rabbit antibody targeting GAP43. Anti-GAP43 Antibody (6G327) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-GAP43 Antibody (6G327)
Anti-GAP43 Antibody (6G327)
😃Good
Catalog No. TMAC-01612Alias PP46, growth associated protein 43, B-50
Anti-GAP43 Antibody (6G327) is a Rabbit antibody targeting GAP43. Anti-GAP43 Antibody (6G327) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-GAP43 Antibody (6G327) is a Rabbit antibody targeting GAP43. Anti-GAP43 Antibody (6G327) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | PP46, growth associated protein 43, B-50 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of GAP43 on mouse brain lysates using anti-GAP43 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GAP43 antibody. Counter stained with hematoxylin. 3. ICC staining GAP43 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining GAP43 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of SH-SY-5y cells with GAP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:2000-5000; IHC: 1:100-500; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | GAP-43 (growth associated protein 43, B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1 is a crucial component for regenerative response in the nervous system. It is present at high levels in neuronal growth cones during development and axonal regeneration. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The neuron-specific Elav/Hu family member, HuD, interacts with and stabilizes GAP-43 mRNA in developing neurons and leads to increased levels of GAP-43 protein. Heterozygous GAP-43 knockout mice with GAP-43 levels reduced by one-half dipslay significant memory impairments in cued conditioning or on tests of nociceptive or auditory perception. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 48 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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